
LAMP and Other Isothermal Amplification Technologies
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Thermo Scientific™ EquiPhi29™ DNA Amplification Kit
The Thermo Scientific EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which includes rolling circle amplification (RCA) and multiple displacement amplification – whole genome amplification (MDA-WGA).
Polymérase | EquiPhi29 DNA Polymerase |
---|---|
Température de réaction optimale | 42°C |
Format | Kit |
Catégorie de recherche | Nucleic Acid Analysis,In Vitro Transcription,Cell-free Protein Expression,In Situ Sequencing,SNP Detection |
Type de produit | DNA Amplification Kit |
Temps de réaction | Up to 2 hours |
Conditions d’expédition | Dry Ice |
À utiliser avec (application) | RCA,MDA-WGA |
Forme | Liquid |
Invitrogen™ SuperScript™ IV RT-LAMP Master Mix
Invitrogen SuperScript IV RT-LAMP Master Mix is a ready-to-use solution optimized for fast, sensitive, and simple RNA target detection in loop-mediated isothermal amplification (LAMP) reactions.
Polymérase | Bst DNA Polymerase |
---|---|
Température de réaction optimale | 65°C |
Type de produit | Master Mix |
Temps de réaction | 5 to 15 minutes |
Concentration | 2X |
Conditions d’expédition | Dry Ice |
Transcriptase inverse | SuperScript IV |
Comprend l’étiquette ou le colorant | SYTO 9 Green Fluorescent Nucleic Acid Stain |
À utiliser avec (application) | LAMP,RT-LAMP |
Thermo Scientific™ EquiPhi29™ DNA Polymerase
Thermo Scientific EquiPhi29 DNA Polymerase is a proprietary phi29 DNA polymerase mutant developed through in vitro protein evolution.
Polymérase | EquiPhi29 DNA Polymerase |
---|---|
Température de réaction optimale | 42°C |
Type de produit | Stand-alone enzyme |
Temps de réaction | 2 hours |
Concentration | 10 U/μL |
Conditions d’expédition | Dry Ice |
À utiliser avec (application) | MDA-WGA,RCA |
Invitrogen™ Colorimetric ReadiLAMP™ Kit, SARS-CoV-2
The Invitrogen Colorimetric ReadiLAMP Kit for SARS-CoV-2 is a loop-mediated isothermal amplification (LAMP)-based solution for rapid SARS-CoV-2 detection in saliva and nasopharyngeal/nasal swab samples.
Enzymes | SuperScript IV & proprietary Bst-derived DNA polymerase |
---|---|
Méthode de détection | Colorimetric readout |
Couleur | During reaction color changes from purple to blue |
À utiliser avec (équipement) | Heating block |
Type de microbilles | Kit does not contain beads. Recommending the use of RNA extraction MVP II kit which contains magnetic beads |
Principales fonctions | Detects SARS-CoV-2 presence |
Limite de détection | <250 copies/25 μL (Direct LAMP) <100 copies/25 μL (Purified RNA-based LAMP) |
Type d’échantillon | Saliva or Nasopharyngeal/Nasal Swab |
Comprend | LAMP Buffer (5x), Enzyme Mix (10x), SARS-CoV-2 primer mix (5x), Human Control primer mix (5x), SARS-CoV-2 control RNA |
Formule | Liquid. 5 components included in the kit. |
Volume d’échantillon | Range of sample inputs |
À utiliser avec (application) | SARS-CoV-2 surveilance |
Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)
Invitrogen Lyo-ready RPA reagents are designed for fast, sensitive, and specific DNA amplification through isothermal recombinase polymerase amplification (RPA) reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats.
Catégorie de recherche | Nucleic Acid Amplification |
---|---|
Conditions d’expédition | Dry Ice |
Forme | Liquid |
Thermo Scientific™ Bsm DNA Polymerase, large fragment (8 U/μL)
Thermo Scientific Bsm DNA Polymerase, Large Fragment, is equivalent to Bst DNA polymerase, which catalyzes 5' → 3' synthesis of DNA and lacks 5' → 3' and 3' → 5' exonuclease activities.
Thermo Scientific™ phi29 DNA Polymerase (10 U/μL)
Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification.
Polymérase | phi29 DNA Polymerase |
---|---|
Température de réaction optimale | 30°C |
Type de produit | Stand-alone enzyme |
Temps de réaction | 4 hours |
Concentration | 10 U/μL |
Conditions d’expédition | Dry Ice |
À utiliser avec (application) | MDA-WGA,RCA |