DNA Polymerases

DNA Polymerases
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Thermo Scientific™ DNA Polymerase I (10 U/μL)
DNA Polymerase I is a template-dependent DNA polymerase which catalyzes 5'→3' synthesis of DNA.
Polymérase | DNA Polymerase I |
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Description | DNA Polymerase I |
Concentration | 10 U/μL |
Invitrogen™ T4 DNA Polymerase
DNA polymerase with 3'-exodeoxyribonuclease activity, but not 5'a3' exodeoxyribonuclease activity
Polymérase | T4 DNA Polymerase |
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Description | DNA Polymerase |
Activité exonucléasique | 3' - 5' |
Hot Start | No |
Conditions d’expédition | Wet Ice |
Contenu et stockage | T4 DNA Polymerase is supplied with a vial of 5X T4 DNA Polymerase buffer [165 mM Tris acetate (pH 7.9), 330 mM sodium acetate, 50 mM magnesium acetate, 5 mM DTT]. Store at -20°C. |
Polymérase | DNA Polymerase I |
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Description | DNA Polymerase |
Activité exonucléasique | 3' - 5', 5' - 3' |
Hot Start | No |
Conditions d’expédition | Wet Ice |
Thermo Scientific™ T4 DNA Polymerase (5 U/μL)
Ensure accuracy in DNA synthesis with template-dependent T4 DNA Polymerase that catalyzes the 5'-3' synthesis from primed single-stranded DNA.
Polymérase | T4 DNA Polymerase |
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MP Biomedicals™ Taq DNA Pol
A thermostable DNA polymerase that is used for PCR, RT-PCR, DNA sequencing and DNA labeling.
Description | Taq DNA Pol |
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Concentration | 5U/μL |
À utiliser avec (application) | Standard PCR,RT-PCR,Sequencing,Nucleic Acid Labeling |
Forme | Liquid |
Contenu et stockage | Store at -20°C |
Cytiva Thermo Sequenase DNA Polymerase
Novel thermostable DNA polymerase that uses dideoxynucleotide triphosphates (ddNTPs) as readily as deoxynucleotide (dNTP) substrates
Description | Thermo Sequenase DNA Polymerase |
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Nbre de réactions | 32 units/μL |
Source | E. coli clones that overproduce the protein |
À utiliser avec (application) | Sequencing,Genotyping |
Contenu et stockage | -20°C, Solution in 20 mM Tris-HCl, pH 8.5, 0.1 mM EDTA, 0.5% Tween 20 (v/v), 0.5% Nonidet P-40 (v/v), 1 mM DTT, 100 mM KCl, 50% glycerol |
MP Biomedicals™ Taq DNA Polymerase (5U/μL)
Includes Direct Loading Buffer for loading of sample onto agarose gel after amplification
Comprend | Taq DNA Polymerase, 5X Direct Loading Buffer with Mg, 25mM MgCl2 |
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Concentration | 5U/μL |
Contenu et stockage | Store at -20°C |
À utiliser avec (application) | Standard PCR,RT-PCR,Sequencing,Nucleic Acid Labeling |
Comprend | Taq DNA Polymerase, 10X Standard Buffer with Mg, 10X Standard Buffer without Mg, 25mM MgCl2 |
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Concentration | 15U/μL |
À utiliser avec (application) | Standard PCR |
MP Biomedicals™ Taq CORE Kit 10
Designed for the amplification of specific regions of DNA using PCR. MP Biomedicals Taq CORE Kit 10 provides the enzymes, buffers and dNTPs necessary for amplification, including the (NH4)2SO4 buffer, which has been report
Promega GoTaq™ G2 Green Master Mix
GoTaq(R) G2 Master Mixes are premixed ready-to-use solutions containing GoTaq(R) G2 DNA Polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of a wide range of DNA templates by PCR.
Description | Green Master Mix |
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À utiliser avec (application) | RT-PCR |
Contenu et stockage | −30° to −10°C |
Promega GoTaq™ Flexi DNA Polymerase
Taq DNA Polymerase. Provided with Green and Colorless Reaction Buffers without MgCl2, and 25mM MgCl2. Green Buffer allows direct gel analysis after amplification. Enhanced performance over standard Taq DNA Polymerase.
Description | DNA Polymerase |
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pH | 8 |
Comprend | GoTaq Flexi DNA Polymerase, 5X Colorless GoTaq Flexi buffer (Used when direct fluorescence or absorbance readings are required without prior purification of the DNA from the amplification reaction), 5X Green GoTaq Flexi buffer, 25mM MgCl2 |
Concentration | 5U/μL |
À utiliser avec (application) | Standard PCR |
Forme | Liquid |
Contenu et stockage | −30 to −10°C |
G-Biosciences Hot Start Taq DNA Polymerase
Hot start Taq DNA Polymerase is used for routine PCR, qPCR, genotyping and high GC content template DNA amplification with GC enhancer.
GC-Rich PCR Performance | High |
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Méthode de détection | qPCR |
Vitesse de réaction | Standard |
Type de produit | DNA Polymerase |
Méthode PCR | Standard PCR,Direct PCR,qPCR,Multiplex qPCR,1-step RT-qPCR |
À utiliser avec (application) | Hot-start PCR,Multiplex PCR,Genotyping,DNA Sequencing,Next-Generation Sequencing (NGS) |
Marqueur ou colorant | Biotin,6-FAM,ROX,SYBR Green,TAMRA |
Forme | Liquid |
Thermo Scientific™ T7 DNA Polymerase (10 U/μL)
Catalyze DNA synthesis in the 5'≥3' direction with T7 DNA Polymerase, a template dependent DNA polymerase.
Invitrogen™ DNA Polymerase I/DNase I
An optimized mixture of both enzymes for efficient nick translation of DNA
Invitrogen™ DNA Polymerase I, Large (Klenow) Fragment
DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I, but does exhibit the 5' to 3' DNA polymerase and 3' to 5' exonuclease activities
Polymérase | DNA Polymerase I |
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Hot Start | No |
Contenu et stockage | DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact™ 2 Buffer [500 mM Tris-HCl (pH 8.0), 100 mM MgCl2 , 500 mM NaCl], vial of dilution buffer. Store at -20°C. |