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  5. DNA Polymerases
  6. Invitrogen™ DNA Polymerase I, Large (Klenow) Fragment

Invitrogen™ DNA Polymerase I, Large (Klenow) Fragment DFS Item

DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I, but does exhibit the 5' to 3' DNA polymerase and 3' to 5' exonuclease activities

$377.00 - $952.00

Spécifications

Content And Storage DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact™ 2 Buffer [500 mM Tris-HCl (pH 8.0), 100 mM
MgCl2 , 500 mM NaCl], vial of dilution buffer. Store at -20°C.
Polymerase DNA Polymerase I
Hot Start No
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Comprend: DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact™ 2 Buffer [500mM Tris-HCl (pH 8.0), 100mM MgCl2, 500mM NaCl], vial of dilution buffer.

Produits 2
Numéro de catalogue Numéro du manufacturier. Quantity Prix Quantité  
Numéro de catalogue Numéro du manufacturier. Quantity Prix Quantité  
18012021 DFS Item
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Invitrogen™
18012021 		100 Units
chaque for $377.00
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18012039 DFS Item
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Invitrogen™
18012039 		500 Units
chaque for $952.00
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Description

Description

Applications:
Fill-in of 5' overhangs . Synthesis of probes by random primers labeling method. Sequencing single- and double-stranded DNA. Site-directed mutagenesis.

Source:
Purified from E. coli expressing the Klenow fragment on a plasmid.

Performance and Quality Testing:
SDS-PAGE purity; single-stranded and double-stranded endodeoxyribonuclease and self-priming assays; performance evaluated in fill-in reaction.

Unit Definition:
One unit incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template•primer.

Array CGH, Cloning, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Restriction Enzyme Cloning

Order Info

Shipping Condition: Approved for shipment on Wet or Dry Ice

Spécifications

Spécifications

DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact™ 2 Buffer [500 mM Tris-HCl (pH 8.0), 100 mM
MgCl2 , 500 mM NaCl], vial of dilution buffer. Store at -20°C.
DNA Polymerase I
No
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For Research Use Only. Not for use in diagnostic procedures.