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Affinity Gels

Gels specifically formulated for use with affinity electrophoresis.

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1

Cytiva Epoxy-activated Sepharose™ 6B Medium

A pre-activated medium for immobilization of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose 6B via a 12-atom hydrophilic spacer arm

2

Cytiva CNBr-Activated Sepharose™ Medium

Use for coupling antibodies or other large proteins containing -NH2 groups to the Sepharose media. CNBr-Activated Sepharose™ Medium is a pre-activated media perfect for custom affinity applications.

3

Cytiva Lentil Lectin Sepharose™ 4B Medium

Routinely used in the preparation and purification of a number of glycoproteins and carbohydrate-containing molecules

4

Cytiva Calmodulin Sepharose™ 4B Medium

Use for purification of proteins with affinity for calmodulin, a highly conserved regulatory protein involved in many cellular processes in eukaryotic cells. Calmodulin Sepharose™ 4B Medium provides single-step purification.

5

Cytiva Con A Sepharose™ 4B Medium

An affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids

Capacité de liaison 30mg/mL
Matrice 4% Agarose
Ligand Concanavalin A
pH 4 to 9
Granulométrie 90 μm
Concentration de ligand 13mg Con A/mL Drained Medium
À utiliser avec (application) For separation and purification of Glycoproteins, Polysaccharides and Glycolipids, isolates cell surface Glycoproteins from detergent-solubilized membranes
Contenu et stockage 2°C to 8°C
6

Cytiva Protein G Sepharose™ 4 Fast Flow Media

Rapid processing of large volumes, recoveries of 70 to 90%. Cytiva Protein G Sepharose™ 4 Fast Flow Media is recombinant protein G coupled to Sepharose 4 Fast Flow.

Matrice 4% Cross Linked Agarose
Ligand Recombinant Protein G lacking albumin binding region
pH 3 to 9
Granulométrie 90 μm
Concentration de ligand 2mg Protein G/mL Medium
À utiliser avec (application) Used in a range of diagnostic and research applications
Contenu et stockage 2°C to 8°C
7
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Cytiva MagRack 6

Use for small-scale sample preparation using magnetic beads. Cytiva Life Sciences™ MagRack 6 enables handling of up to six samples in 1.5 ml microcentrifuge tubes.

8

Cytiva NHS Mag Sepharose™

NHS Mag Sepharose magnetic beads for convenient enrichment of selected proteins using pull-down sample preparation techniques

Matrice Paramagnetic spherical, highly cross linked agarose particles
Ligand N-hydroxysuccinimide
Type de microbilles NHS Mag Sepharose
Granulométrie 37 to 100 μm
Concentration de ligand 8 to 14μM/mL
Contenu et stockage 4°C to 8°C
9

Cytiva Protein A Mag Sepharose™ Xtra

Magnetic beads are designed for rapid, small-scale purification and screening of monoclonal and polyclonal antibodies from serum and cell supernatants

Capacité de liaison 27mg human IgG/mL gel
10

Cytiva EAH Sepharose™ 4B Medium

Used for coupling compounds containing carboxyl groups to Sepharose 4B. EAH Sepharose™ 4B Medium provides extremely stable coupling via an 11-atom spacer arm.

11

Cytiva HiLoad™ Superdex™ 75 pg

For high resolution preparative gel filtration chromatography with short run times and good recovery. Cytiva Lifescience™ HiLoad™ Superdex™ 75 pg are prepacked XK columns.

À utiliser avec (application) high resolution preparative gel filtration chromatography
12

Cytiva Protein A and Protein G Mag Sepharose™

Simplify enrichment of target proteins by immunoprecipitation or pull-down applications

Matrice Paramagnetic spherical, highly cross linked agarose particles
Granulométrie 37 to 100 μm
Contenu et stockage 4°C to 8°C
13

Cytiva HiLoad™ 16/600 Superdex™ 200 pg

Use for separating MAbs from dimers and contaminants. Cytiva HiLoad™ 16/600 Superdex™ 200 pg is ideal for high-resolution preparative gel filtration chromatography.

14

Cytiva Protein G Mag Sepharose™ Xtra

High binding capacity, 27mg human IgG/mL medium, for efficient capture of antibody with high yield and purity

Capacité de liaison 27mg human IgG/mL gel
Matrice Paramagnetic, spherical, highly cross-linked agarose particles
Ligand Protein G
Type de microbilles Protein G Mag Sepharose Xtra magnetic bead
À utiliser avec (application) For rapid, small-scale purification and screening of monoclonal and polyclonal antibodies from serum and cell supernatants
Contenu et stockage 4°C to 8°C
15

Cytiva HiLoad™ 26/600 Superdex™ 200 pg

Use for separating MAbs from dimers and contaminants. Cytiva Life Sciences™ HiLoad™ 26/600 Superdex™ 200 pg is ideal for high-resolution preparative gel filtration chromatography.