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Direct PCR Reagents and Kits

Direct PCR Reagents and Kits

PCR products that allow for the amplification of DNA directly from unpurified source material. Includes various kits, master mixes, enzymes, and reagents.
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1 – 15 of 61 results
1

Thermo Scientific™ Phire Plant Direct PCR Master Mix

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.

Shipping Condition Wet or Dry Ice
Polymerase Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance Medium
Reaction Format SuperMix or Master Mix
Product Type Direct PCR Master Mix
Sample Type Plant
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
Starting Material DNA
For Use With (Application) Genotyping,Hot-start PCR
PCR Method Direct PCR
2

Thermo Scientific™ Phire Tissue Direct PCR Master Mix

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.

Shipping Condition Wet or Dry Ice
GC-Rich PCR Performance Medium
Includes Label or Dye Yes
Overhang Blunt
Concentration 2X
For Use With (Application) Genotyping,Hot-start PCR
PCR Method Direct PCR
Polymerase Phire Hot Start II DNA Polymerase
Reaction Format SuperMix or Master Mix
Product Type Direct PCR Master Mix
Sample Type Tissue
Reaction Speed Fast
Hot Start Built-In Hot Start
Starting Material DNA
4

Invitrogen™ CellsDirect Resuspension & Lysis Buffers includes 10 ml Resuspension Buffer & 1 ml Lysis Buffer  DFS Item

These two buffers are to be used only as a complement to the SuperScript™ III Platinum™ CellsDirect Kits when large number of samples are processed.

5

Invitrogen™ TaqMan™ Cells-to-CT™ Express Lysis Reagents
GREENER_CHOICE

This TaqMan Cells-to-CT Express Lysis Reagents product is a convenient, large volume offering of the cell lysis reagents used in our TaqMan Cells-to-CT Express Kit.

6

Invitrogen™ Single Cell Lysis Kit  DFS Item
GREENER_CHOICE

Single-cell analysis has the potential to transform life science research. However, detecting and quantitating the minute amounts of RNA contained in single cells remains a significant challenge.

7

Thermo Scientific™ GeneJET PCR Purification Kit

Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

Content And Storage For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
Format Spin Column
Run Time 5 min.
High-throughput Compatibility Not High-throughput Compatible (Manual)
8

Thermo Scientific™ Phire Animal Tissue Direct PCR Kit (without sampling tools)

Thermo Scientific Phire Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects.

11

Invitrogen™ TaqMan™ PreAmp Cells-to-CT™ Kit  DFS Item
GREENER_CHOICE

The TaqMan PreAmp Cells-to-CT Kit makes it possible to perform gene expression analysis directly from limited or small numbers of cultured cells without RNA purification.

12

Invitrogen™ TaqMan™ Cells-to-CT™ Control Kit  DFS Item
GREENER_CHOICE

The Invitrogen™ TaqMan™ Cells-to-CT Control Kit is designed for use with the TaqMan™ Gene Expression Cells-to-CT Kit.

13

Invitrogen™ Cells-to-CT™ Stop Solution  DFS Item
GREENER_CHOICE

A component of the Cells-to-CT™ Kits.

14

Invitrogen™ Cells-to-CT Bulk RT Reagents  DFS Item
GREENER_CHOICE

Cells-to-CT Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription in the TaqMan™ Gene Expression Cells-to-CT™ Kit (20X RT enzyme mix and 20X RT buffer).

15

Invitrogen™ Power SYBR™ Green Cells-to-CT™ Kit  DFS Item
GREENER_CHOICE

Ambion™ Power SYBR™ Green Cells-to-CT™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results.

Shipping Condition Dry Ice
Purity or Quality Grade LD (Low DNA)
Reverse Transcriptase M-MLV
Purification Time 5 min.
Green Features Fewer resources used and less waste, Less hazardous
Concentration 20X
For Use With (Application) RT-PCR
Detection Method SYBR
Polymerase AmpliTaq Gold DNA Polymerase
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7200 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 5700, GeneAmp 9600, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Cells
Reaction Speed Standard
Label or Dye SYBR Green
Hot Start Built-In Hot Start
Product Line Cells-to-CT™, SYBR™