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Invitrogen™ TaqMan™ Fast Cells-to-CT™ Kit 
The TaqMan™ Fast Cells-to-CT™ Kit makes it possible to use Fast-cycling, real-time PCR chemistry and instrumentation to perform gene expression analysis directly from cultured cells without RNA purification.
Supplier: Invitrogen™ 4399003
Includes: Stop Solution: 500μL tube; DNase I: 55μL tube; 20X RT Enzyme Mix: 275μL tube; Lysis Solution: 5.5mL bottle; 2X RT Buffer: 5.5mL bottle; TaqMan Fast Universal PCR Master Mix: 4 x 1.25mL bottle
Description
- Complete: Optimized workflow includes cell lysis reagents with gDNA removal, RT enzyme mix, RT buffer, and TaqMan™ Fast Universal PCR Master Mix
- Fast: Prepare samples at room temperature in 7 minutes, including DNase treatment; TaqMan™ Fast Universal PCR Master Mix delivers real-time PCR results in ∼35 minutes
- Convenient: Lyse samples in tube or directly in 96- or 384-well culture plates
- Robust: Perform gene expression analysis on 10 to 100,000 cells per sample, with results equivalent to those from purified RNA
- Efficient: Contains sufficient reagents to generate 500 real-time PCR results from 100 starting samples
Order Info
Shipping Condition: Dry ice
Specifications
Contains: |
|
| Tube | |
| 2-step RT-qPCR | |
| Fast | |
| Fewer resources used and less waste, Less hazardous | |
| Ambion™, Cells-to-CT™, TaqMan™ | |
| 100 reactions | |
| Dry Ice | |
| Gene Expression | |
| Cell |
| Primer-probe | |
| Low | |
| DNA Polymerase | |
| 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode | |
| ROX (Pre-mixed) | |
| Fast Cells-to-C Kit | |
| M-MLV | |
| 100 Reactions | |
| 100 reactions |
For Research Use Only. Not for use in diagnostic procedures.