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HPLC Columns

HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
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Phase 
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Diameter (Metric)

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Length (Metric)

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Particle Size

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Phase

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Pore Size

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Product Line

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Technique

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Carbon Load

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Material

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Type

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1 – 15 of 940 results
1

Thermo Scientific™ Acclaim™ C30 HPLC Columns

Use for superior separation of hydrophobic structural isomers.

Pore Size 200 Å
Carbon Load 13%
Stationary Phase C30
Temperature 60°C
Phase Reversed Phase
Column Type Reversed Phase
pH 2 to 8
Surface Area 200 m²/g
Product Line Acclaim
2

Thermo Scientific™ Hypersil™ ODS C18 Columns

Use as a general purpose C18 phase for acidic and neutral analytes.

Pore Size 120 Å
Carbon Load 10%
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
pH 2 to 8
Surface Area 170 m²/g
USP Type L1
3

MilliporeSigma™ Supelco™ Ascentis™ Express F5 HPLC Column, 2.7 μm Particle Size

Ascentis™ Express F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

4

Thermo Scientific™ Aquasil™ 5μm C18 HPLC Columns, 5?m Particle Size

Thermo ScientificTM AquasilTM 5μm C18 HPLC Columns are available in limited dimensions. Please contact Customer Service for more details.

Pore Size 100 Å
Particle Size 5 μm
Carbon Load 12%
Stationary Phase C18
Phase Reversed Phase
For Use With LC/MS
Endcapped Yes
Packing Material Silica
Column Type Reversed Phase
pH 2 to 8
Surface Area 310 m²/g
USP Type L1
Column Format Analytical Column
5

Thermo Scientific™ Acclaim™ Polar Advantage II HPLC Columns

Resolve polar and nonpolar compounds in a single run. These columns have high pH stability (pH 1.5–10) in the separation of basic, neutral, and acidic species.

Pore Size 120 Å
Stationary Phase Polar Embedded
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Packing Material Spherical, Fully Porous, Ultrapure Silica
Column Type Reversed Phase
Surface Area 300 m²/g
USP Type L60
6

Thermo Scientific™ SurePac™ Bio 550 SEC MDi™ HPLC Columns

Experience the future of Size-Exclusion Chromatography (SEC) with SurePac™ Bio 550 SEC MDi™ analytical columns and guards exclusively designed to deliver robust and reproducible performance at high resolution and throughput to accelerate your therapeutics analysis process.

Particle Size 3 μm
Stationary Phase Proprietary
Temperature 20°C to 60°C
Phase Size Exclusion
Packing Material Monodisperse Silica Resin
pH 2 to 8
Quantity 1 Ea.
Technique SEC
7

MilliporeSigma™ Supelco™ Zenix™ SEC-100 Gel Filtration HPLC Column, 3 μm Particle Size, Stainless Steel

Like their 5 micron siblings, 3 micron Zenix™ SEC columns feature a hydrophilic surface layer to prevent the sample from interacting with the underlying silica.

Pore Size 100 Å
Particle Size 3 μm
Stationary Phase Hydrophilic Polymer
Phase Size Exclusion
Matrix Hydrophilic Polymer Phase/Spherical Silica
Length (Metric) 30 cm
pH 2.0 to 8.5
Material Stainless Steel
Technique SEC HPLC
Product Line Zenix
8

MilliporeSigma™ Supelco™ Zenix™ SEC-300 Gel Filtration HPLC Column, 3 μm Particle Size, Stainless Steel

Like their 5 micron siblings, 3 micron Zenix™ SEC columns feature a hydrophilic surface layer to prevent the sample from interacting with the underlying silica.

Pore Size 300 Å
Particle Size 3 μm
Stationary Phase Hydrophilic Polymer
Phase Size Exclusion
Matrix Hydrophilic Polymer Phase/Spherical Silica
pH 2.0 to 8.5
Material Stainless Steel
Technique SEC HPLC
Product Line Zenix
9

MilliporeSigma™ Supelco™ Zenix™ SEC-150 Gel Filtration HPLC Column, 3 μm Particle Size, Stainless Steel

Like their 5 micron siblings, 3 micron Zenix™ SEC columns feature a hydrophilic surface layer to prevent the sample from interacting with the underlying silica.

Pore Size 150 Å
Particle Size 3 μm
Stationary Phase Hydrophilic Polymer
Phase Size Exclusion
Matrix Hydrophilic Polymer Phase/Spherical Silica
Length (Metric) 30 cm
pH 2.0 to 8.5
Material Stainless Steel
Technique SEC HPLC
Product Line Zenix
10

Thermo Scientific™ MAbPac™ SEC-1 Size Exclusion Chromatography HPLC Columns

Use these SEC columns for monoclonal antibody (mAb) analysis, including the high-resolution separation of monomers, aggregates and fragments.

Pore Size 300 Å
Particle Size 5 μm
Solvents 100% Organic Solvents
Temperature 30°C
Phase Size Exclusion
Column Type Size Exclusion
pH 2.5 to 7.5
Quantity Each
Green Features Responsibly packaged
Product Line MAbPac
12

MilliporeSigma™ Supelco™ Nucleosil™ C18 HPLC Column, 3 μm Particle Size

Nucleosil™ C18 HPLC Column features nonpolar, endcapped phases for reversed-phase chromatography.

13

MilliporeSigma™ Supelco™ TSKgel™ G3000SWXL HPLC Column, 5 μm Particle Size, Sold by MilliporeSigma™ Supelco™

Widely considered the premium columns for analyzing biopolymers by GFC, TSKgel™ SWXL columns feature high pore volume per unit column volume, low sample adsorption and excellent column efficiency, all contributing to unsurpassed sample resolution.

14

Thermo Scientific™ DNAPac™ RP Columns

Get high resolution LC and LC-MS separation of oligonucleotides, including double-stranded (ds) DNA/RNA fragments, using these DNAPac reversed phase columns.

15

Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns

Use these endcapped, ultrapure, silica-based columns to generate exceptional peak shape and resolution for HPLC and LC/MS analyses using generic gradients