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Thermo Scientific™ Phire Tissue Direct PCR Master Mix
Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.
Supplier: Thermo Scientific™ F170S
Description
Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, humans, fish, birds, and insects. The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue–derived inhibitors such as collagen, melanin and eumelanin (hair, skin), and myoglobin (muscle). The kit also includes DNARelease Additive, which can be used to improve the release of DNA from difficult tissues.
Features
- Direct PCR reduces time-consuming and expensive DNA purification steps
- Available Dilution & Storage protocol allows multiple PCR reactions from a single sample
- Specially engineered Phire DNA polymerase for extremely short PCR protocol times
- Master mix format with premixed gel loading dye minimizes cross-contamination, reduces sample handling, and allows direct loading on gel
Applications
- Genotyping
- Transgene detection
- Knockout analysis
- Sequencing
Notes
- The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.
Specifications
| • 2X Phire Tissue Direct PCR Master Mix, 2 x 1.25 mL • Dilution Buffer, 5 mL • DNARelease Additive, 3 x 100 μL • 25 μM Control Primer Mix, 40 μL • Nuclease-free water, 2 x 1.25 mL • 50 μg O'GeneRuler Express DNA Ladder Store at -20°C. |
|
| Direct PCR | |
| Fast | |
| Direct PCR Master Mix | |
| Wet or Dry Ice | |
| 2X | |
| 250 Reactions | |
| SuperMix or Master Mix | |
| DNA |
| Medium | |
| Phire Hot Start II DNA Polymerase | |
| Yes | |
| 250 reactions | |
| Genotyping, Hot-start PCR | |
| Built-In Hot Start | |
| Blunt | |
| Tissue |
For Research Use Only. Not for use in diagnostic procedures.