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Invitrogen™ Quant-it™ RiboGreen Reagent and RNA Assay Kit 
Quant-iT RiboGreen RNA Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. The Quant-iT RiboGreen RNA Assay Kit comes with reagent, dilution buffer, and rRNA standards to provide a linear detection range of 1â200 ng of RNA.
$443.00 - $1,124.00
Specifications
| Detection Method | Fluorescence |
|---|---|
| Excitation/Emission | 500/525 |
| For Use With (Equipment) | Microplate Reader |
| Shipping Condition | Room Temperature |
| Catalog Number | Mfr. No. | Quantity | Product Type | Quantitation Range | Price | Quantity | |||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Quantity | Product Type | Quantitation Range | Price | Quantity | |||
|
R11490
|
Invitrogen™
R11490 |
1 kit | Quant-iT RiboGreen RNA Kit | 1 to 200 ng |
N/A
|
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R11491
|
Invitrogen™
R11491 |
1 mL | Quant-iT RiboGreen RNA Reagent | 1 to 200 ng |
N/A
|
|
|||
|
R32700
|
Invitrogen™
R32700 |
1 kit | RediPlate 96 RiboGreen RNA Kit | 3 to 200 ng |
N/A
|
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|||
Description
Quant-iT RiboGreen RNA Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. The Quant-iT RiboGreen RNA Assay Kit comes with reagent, dilution buffer, and rRNA standards to provide a linear detection range of 1â200 ng of RNA.
Workflow
This easy-to-use reagent offers two detection ranges: a high-assay range and a low-assay range for sensitive and broad range detection. To use, simply prepare the working solution by diluting the provided dye with TE-Buffer, then aliquotting 100 µL of the working solution with standards and samples for a total reaction volume of 200 µL. RNA is then quantitated using a fluorescence microplate reader (such as Varioskan ALF) at standard fluorescein wavelengths.
Applications
Detecting and quantitating small amounts of RNA is important in many applications including measuring yields of in vitro transcribed RNA and measuring RNA concentrations before performing Northern blot analyses, S1 nuclease assays, RNase protection assays, cDNA library preparation, reverse transcription PCR, and differential display PCR.
Specifications
| Fluorescence | |
| 500/525 | |
| Microplate Reader | |
| Room Temperature |