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Thermo Scientific™ Sulfo-LC-SPDP (sulfosuccinimidyl 6-(3'-(2-pyridyldithio)propionamido)hexanoate)
Crosslink amines to sulfhydryls with this water-soluble sulfo-NHS-ester and pyridyldithiol groups on 10-atom spacer arms to form cleavable disulfide bonds with cysteines.
Supplier: Thermo Scientific™ 21650
Description
Thermo Scientific™ Pierce Sulfo-LC-SPDP is a water-soluble, long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteines.
Highlights:
- Forms cleavable disulfide bonds with sulfhydryls
- Releases a detectable byproduct after reacting with free sulfhydryl groups; by measuring the release of pyridine-2-thione at 343nm, the reaction progression can be monitored
- Reactive groups: sulfo-NHS ester and pyridyldisulfide
- Reactive toward: amino and sulfhydryl groups
Specifications
| Amine-Sulfhydryl | |
| Sulfo-LC-SPDP | |
| No | |
| Upon receipt store at -20°C protected from moisture. | |
| Ambient | |
| Chemical Labeling | |
| Pyridyldisulfide, Sulfo-NHS Ester | |
| Powder | |
| Water | |
| Yes |
| By Thiols | |
| 527.57 | |
| 15.7 AÌ | |
| No | |
| Pierce™ | |
| Heterobifunctional | |
| Medium (10 to 30 AÌ) | |
| 50 mg | |
| Standard | |
| Crosslinker |
For Research Use Only. Not for use in diagnostic procedures.