missing translation for 'onlineSavingsMsg'
Learn More
  1. Acceuil
  2. Produits
  3. Cell Culture Media, Supplements, and Reagents
  4. Cell Culture Media
  5. Classical and Basal Cell Culture Media
  6. DMEM-F-12 Cell Culture Media
  7. Gibco™ DMEM/F-12
  8. 11320082

Gibco™ DMEM/F-12 DFS Item

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Supplier:  Gibco™ 11320082

 Afficher plus de versions de ce produit

Numéro de catalogue. 11320082


Il en reste null
Découvrez plus d'offres spéciales

Description

Description

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM/F-12 is modified as follows:

With: L-glutamine, Phenol Red

Without: HEPES

The complete formulation is available.

Using DMEM/F-12

DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Spécifications

Spécifications

MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Animal Origin-free
Liquid
Standard Serum Supplementation
Sterile-filtered
cGMP-compliant under the ISO 13485 standard
10 x 500 mL
Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
1 X
DMEM (Dulbecco's Modified Eagle Medium)/F-12
Sterile-filtered
High Glucose, Glutamine, Phenol Red, Sodium Pyruvate
Gibco™
Room Temperature
missing translation for 'recommendations'

missing translation for 'recommendations'

Videos
FDS
Documents

Documents

Promotions

Promotions

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.