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Applied Biosystems™ BigDye™ Direct Cycle Sequencing Kit 
The BigDye™ Direct Cycle Sequencing Kit, part of the BigDye™ product family, is a complete kit for your PCR and Sequencing needs.
Supplier: Applied Biosystems™ 4458687
Comprend: This kit contains BigDye™ Direct PCR Master Mix, Sequencing Master Mix, M13 Fwd Primer, M13 Rev Primer, and Control DNA CEPH 1347-02, This kit contains sufficient reagents for 24 reactions
Description
- A streamlined, simple procedure
- Performance of all sequencing steps in a single reaction tube
- Fast migration with excellent resolution starting at the 1st base after the gene-specific primer sequence
- Substantial time savings versus previous workflows
Order Info
1 Kit
Spécifications
| This kit contains BigDye™ Direct PCR Master Mix, BigDye™ Direct Sequencing Master Mix, BigDye™ Direct M13 Fwd Primer, BigDye™ Direct M13 Rev Primer, and Control DNA CEPH 1347-02. Store all components at -15 to -25°C and protect from light. PCR Master Mix should be stored at 4°C after first use. This kit contains sufficient reagents for 100 reactions. | |
| 3130xl Genetic Analyzer, 3730xl DNA Analyzer, GeneAmp 9700, SeqStudio Genetic Analyzer, Veriti Thermal Cycler, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer | |
| BigDye™ Direct Cycle Sequencing Kit, 100 rxns, 8-well Strips, 96-well Plate, Fluorescent Dye Terminator Sequencing Technique, 3, Use with 130xl Genetic Analyzer, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer, 3730xl DNA Analyzer, GeneAmp™ 9700, Veriti™ Thermal Cycler | |
| BigDye™ | |
| 100 reactions | |
| Fluorescent Dye Terminator Sequencing |
| 8-well Strip, 96-well Plate | |
| Comparative Sequencing (Germline Mutations - 30:70 Heterozygotes), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), GT-Rich or Difficult Template Sequencing, Standard Sequencing, Gap Closure, Homopolymer A or T, AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations -10:90 Heterozygotes) | |
| 100 Reactions | |
| Direct Cycle Sequencing Kit | |
| Dry Ice | |
| BAC DNA, Cosmids, Lambda DNA, PCR Amplicons (10:90 Heterozygotes), Plasmid DNA (≤15Kb), Genomic DNA (Bacterial), Fosmids, PCR Amplicons (50:50 Heterozygotes), PCR Amplicons (30:70 Heterozygotes) |
For Research Use Only. Not for use in diagnostic procedures.