Miscellaneous Molecular Biology Reagents
Invitrogen™ EDTA (0.5 M), pH 8.0, RNase-free
Invitrogen Molecular biology grade, 0.5 M EDTA, pH 8.0 solution is supplied in one bottle. The solution is certified RNase-free, economical, and ready-to-use.
Invitrogen™ UltraPure™ BSA (50 mg/mL)
Ambion Ultrapure BSA is a 'non-acetylated' BSA pure enough to use when DNA or RNA integrity is critical.
Thermo Scientific™ DTT (dithiothreitol)
Stabilize enzymes and other proteins which possess free sulfhydryl groups with DTT, shown to restore activity lost by oxidation of these groups in vitro.
Thermo Scientific™ X-Gal Solution, ready-to-use
Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.
Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)
UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) is used in the purification of nucleic acids. This reagent consists of highly pure chloroform, isoamyl alcohol, and UltraPure™ Phenol saturated with Tris-HCl.
Invitrogen™ UltraPure™ Glycerol
UltraPure Glycerol is suitable for the concentration and storage of enzymes at low temperatures. A 50% (v/v) solution will not freeze at -20°C.
Invitrogen™ UltraPure™ Dithiothreitol
UltraPure Dithiothreitol (DTT, Cleland's reagent), which reduces disulfides to their corresponding thiols, is used at low concentrations to stabilize enzymes containing free sulfhydryl groups.
Invitrogen™ UltraPure™ Cesium Chloride, Optical Grade
UltraPure™ Cesium Chloride, Optical Grade is specifically prepared for use in density gradients.
Invitrogen™ IPTG
IPTG (isopropylthio-β-galactoside), a structural non-metabolizable analogue of allolactose, is an inducer of β-galactosidase activity in bacteria and is suitable for use with X-Gal or Bluo-Gal to detect lac gene activity in cloning procedures.
Invitrogen™ UltraPure™ Buffer-Saturated Phenol
UltraPure™ Buffer-Saturated Phenol is used in the purification of nucleic acids. The reagent, which consists of UltraPure™ Phenol that has been saturated with Tris-HCl buffer, is already buffer equilibrated to pH >7.4.
Invitrogen™ Guanidine Isothiocyanate
UltraPure Guanidine Isothiocyanate is a strong protein denaturant when used at high concentrations.
Invitrogen™ UltraPure™ Formamide
UltraPure Formamide is commonly used for denaturing nucleic acids for sequencing gel electrophoresis, electron microscopy, and hybridization.
Invitrogen™ UltraPure™ Guanidine Hydrochloride
UltraPure Guanidine Hydrochloride is a strong protein denaturant. In a highly concentrated (5 to 6M) guanidine hydrochloride solution, many proteins unfold and separate into their constituent polypeptide chains.
Invitrogen™ X-Gal
X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) is a histochemical substrate for β-galactosidase. This substrate yields a blue precipitate upon hydrolysis, making it suitable for use in immunoblotting or immunocytochemical assays.
Invitrogen™ UltraPure™ Guanidine Isothiocyanate Solution
UltraPure Guanidine Isothiocyanate Solution consists of 4M guanidine isothiocyanate, 50 mM Tris-HCl (pH 7.5), 25 mM EDTA.