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Gel Filtration Media and Columns

Gel Filtration Media and Columns

A variety of columns and media for the size-based separation and purification of proteins, lipoproteins, peptides, etc. using gel filtration chromatography techniques.
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1 – 15 of 44 results
1

Cytiva HiLoad™ 16/600 Superose™ 6 Prep Grade Preparative SEC Column

A prepacked SEC column for high-resolution purification of large biomolecules and protein complexes with sample volumes up to 5 mL

2

Cytiva Sephadex™ G50 Superfine Media

Use as a well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. Sephadex™ G50 Superfine Media's small bead size gives higher efficiency.

3

Cytiva Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

4

Cytiva Sephadex™ G15 Media

Gel filtration medium for desalting and buffer exchange for small biomolecules greater than 1500 molecular weight

5

Cytiva HiTrap™ Capto™ Core 400

Designed for intermediate purification and polishing of viruses and other large biomolecules

6

Cytiva Sephadex G-50 Medium, 500g

Suitable for large scale separation. Cytiva Sephadex G-50 Medium, 500g is a well-established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight.

7

Cytiva Sephadex™ G50 Medium Media

Suitable for gel filtration

Particle Size 100 to 300 μm in wet form, 50 to 150 μm in dry form
Height (Metric) 10cm
Bed Volume 9 to 11mg/g
Format Solid Bead
pH Range 2 to 10
Product Type Sephadex G50 Media
Sample Type DNA
Diameter (Metric) Inner 5cm
Support Type Cross-linked Dextran
Height (English) 3.93 in.
8

Cytiva Superdex 30 Increase Precision 3.2/300 GL Prepacked SEC Column

Designed for high resolution in small-scale preparative purification and analysis for sample volumes up to 500μL

9

Cytiva Sephadex™ G10 Media

Gel filtration medium for desalting and buffer exchange of peptides and small biomolecules

Height (Metric) 30cm
Content And Storage 4 to 30°C
Bed Volume 2 to 3mL/g in Distilled Water
pH Range 2 to 13
Diameter (Metric) Inner 2.6cm
For Use With (Application) Desalting and Buffer exchange of Peptides and small biomolecules >700 M.W.
Max. Pressure 24-49 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d
Particle Size 55 to 165 μm in wet form, 40 to 120 μm in dry form
Flow Rate < Darcy´s Law
Format Solid Bead
Product Type Sephadex G10 Media
Sample Type Peptides, Small Biomolecules
Support Type Cross-linked Dextran
Height (English) 11.81 in.
10

Cytiva Sephadex™ G-100 Media

Commonly used for desalting and buffer exchange of very large biomolecules

Content And Storage 4° to 30°C
Bed Volume 15 to 20mL/g in Distilled Water
Format Solid Bead
pH Range 2 to 10
Product Type Sephadex G-100 Media
Sample Type Large biomolecules
Quantity 100 g
For Use With (Application) Useful for desalting and Buffer exchange of very large biomolecules
Support Type Cross-linked Dextran
Max. Pressure 0.096 bar
11

Cytiva Sephadex™ Ion Exchange Media

Separate molecules over broad molecular weight and pH ranges

Height (Metric) 5 to 15cm
Content And Storage 4° to 30°C
Format Solid Spherical Bead
Product Type Sephadex Ion Exchange Media
For Use With (Equipment) Column or Batch Techniques
For Use With (Application) Separating and purifying Proteins, Polypeptides, Nucleic Acids and other charged biomolecules by using column or batch techniques
Support Type Cross-linked Dextran
Height (English) 2 to 5.9 in.
12

Cytiva Superose™ Media Prep Grade Lab Packs

High performance gel filtration media for scaleup to preparative purifications of biomolecules

Particle Size 30 to 40 μm
Flow Rate 40 cm/hr.
Bed Volume ≈100 mL
pH Range 3 to 12
For Use With (Equipment) AKTA™ design pump or system
Sample Type Proteins, Peptides, Polynucleotides, Wide Variety of Biomolecules
Quantity Each
For Use With (Application) Separations of Proteins and other biomolecules according to size
Support Type Highly Cross-linked Agarose
14

Cytiva Sephadex™ G-25 DNA Grade SF Cleanup Gel

Tested to ensure reproducibly high recovery of DNA (10 bases in length) from small molecules by gel filtration

15

Cytiva HiTrap™ NHS-Activated HP Columns

Couple ligands containing primary amines easily. Cytiva Life Sciences™ HiTrap™ NHS-Activated HP Columns are prepacked with NHS activated Sepharose™ High Performance.

Particle Size 34 μm
Ligand Type 10μM NHS groups/mL medium
Height (Metric) 2.5cm
Content And Storage 4° to 8°C
pH Range 3 to 12
Product Type NHS-Activated HP Column
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Material Polypropylene
For Use With (Application) Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.