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PCR Cloning Kits

PCR Cloning Kits

Chemical reagents, kits, and other products used for polymerase chain reaction (PCR) cloning.
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1

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ TOP10 chemically competent E. coli cells  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for subcloning

Souche bactérienne ou de levures TOP10
Format Kit
Type de produit Cloning Kit
Type de cellule Chemically Competent
Méthode de clonage TOPO™-TA
Contenu et stockage Box 1:
• Topoisomerase I-activated pCR™2.1-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
2

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, without competent cells  DFS Item

Provides a highly efficient, 5-minute cloning reaction for the direct insertion of proofreading polymerase-amplified, blunt-ended PCR products into a plasmid vector for sequencing

Résistance aux antibiotiques des bactéries Ampicillin (AmpR), Kanamycin (KanR)
Format Kit
Type de produit PCR Cloning Kit
Vecteur TOPO Cloning Vectors
Gamme de produits TOPO™
Conditions d’expédition Dry Ice
Accélérateur T7, T3
Méthode de clonage Blunt TOPO™
Contenu et stockage Box 1:
• Topoisomerase I-activated pCR™4Blunt-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• T3, T7, M13 forward, and M13 reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
3

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli cells  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector

Souche bactérienne ou de levures TOP10
Format Kit
Type de produit PCR Cloning Kit
Type de cellule Chemically Competent
Accélérateur SP6
Méthode de clonage Blunt TOPO™
Contenu et stockage Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
4

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, without competent cells  DFS Item

Résistance aux antibiotiques des bactéries Kanamycin (KanR), Zeocin™ (ZeoR)
Format Kit
Type de produit PCR Cloning Kit
Accélérateur SP6
Méthode de clonage Blunt TOPO™
Contenu et stockage Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
6

Invitrogen™ Zero Blunt™ PCR Cloning Kit  DFS Item

Offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proofreading, thermostable DNA polymerases

Souche bactérienne ou de levures TOP10
Format Kit
Type de produit PCR Cloning Kit
Vecteur Blunt DNA Cloning Vectors
Gamme de produits Zero Blunt™
Accélérateur T7
Méthode de clonage Blunt PCR
Contenu et stockage Zero Blunt™ PCR cloning kits contain linearized pCR™-Blunt vector, ExpressLink™ T4 DNA ligase , 5X ExpressLink™ T4 DNA ligase buffer, control template, dNTPs, sterile water, and M13 forward and reverse primers. Competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.

Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
9

Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit  DFS Item

Used in suspension-adapted cells for transient protein expression. Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression.

11

Invitrogen™ PCR Cloning System with Gateway™ Technology with pDONR™/Zeo & OmniMAX™2 Competent Cells  DFS Item

Enables rapid cloning of one or more genes into virtually any protein expression system

12

Invitrogen™ PCR Cloning System with Gateway™ Technology with pDONR™221 & OmniMAX™2 Competent Cells  DFS Item

Enables rapid cloning of one or more genes into virtually any protein expression system

13

Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, without competent cells  DFS Item

Résistance aux antibiotiques des bactéries Ampicillin (AmpR), Kanamycin (KanR)
Format Kit
Vecteur TOPO-TA Vectors
Gamme de produits TOPO™
Accélérateur T7, SP6
Méthode de clonage TOPO™-TA
Contenu et stockage Box 1:
• Topoisomerase I-activated pCR™II-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
14

Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, without competent cells  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing

15

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, without competent cells  DFS Item

Résistance aux antibiotiques des bactéries Ampicillin (AmpR), Kanamycin (KanR)
Format Kit
Type de produit Cloning Kit
Gamme de produits TOPO™
Méthode de clonage TOPO™-TA