Buffer and diluent solutions to prepare, dilute, and deliver protein and nucleic acid samples to electrophoresis gels for separation and analysis; includes solutions to stabilize, control the concentration of, denature, and reduce sample proteins.
Used to prepare protein samples for denaturing gel electrophoresis with NuPAGE™ Novex™ gels. Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X) contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.
Corning™'s 3D Clear Washing Buffer 10X is used for the Corning™ 3D Clear immunolabeling process. This solution is a concentrate and is comprised of: PPBS with 0.2% Tween-20 and 10 μg/mL heparin. This solution should be diluted to 1X concentration with PBS prior to use.
Corning™'s 3D Clear Penetration Buffer is used with the Corning™ 3D immunolabeling process and is comprised of PBS/0.2% Triton™ X-100/0.3 M glycine/20% DMSO.
Corning™'s 3D Clear Antibody Buffer is used with immunolabeling in the Corning™ 3D tissue labeling approach. This solution is comprised of PBS/0.2% Tween 20/Heparin/3% donkey serum/5% DMSO.
Corning™'s 3D Clear Starter Kit combines 30 mL of 3D Clear with the four buffer solutions required to process 3D tissue cultures for immunolabeling. Only immunolabels and nuclear stains need to be added. This kit comes with enough material to process three 96-well plates of 3D cell culture models.