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Vimentin Monoclonal Antibody (V9), Alexa Fluor™ 420, Invitrogen™
Mouse Monoclonal Antibody
Supplier: Fisher Chemical 758989782
Description
Description: The V9 monoclonal antibody recognizes human Vimentin, a 57 kDa protein that functions as a structural component of intermediate filaments. Vimentin is expressed in cells derived from the mesenchyme but also in specific populations such as radial glia and immature glial cells, pancreatic precursor cells. It is proposed to be a marker of cardiac differentiation. In neural cells, vimentin expression is gradually replaced by neurofilaments. Reports have also shown surface expression of vimentin on activated macrophages, platelets, as well as apoptotic T cells and neutrophils. This antibody also recognizes canine (dog), rat and chicken vimentin but does not recognize mouse vimentin. This V9 antibody has been reported for use in western blotting, immunocytochemistry, and immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded tissue sections (IHC-P). This V9 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using high pH antigen retrieval and can be used at 2.5 μg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest.
Vimentin Monoclonal antibody specifically detects Vimentin in Human samples. It is validated for Immunohistochemistry (Paraffin)Specifications
| Vimentin | |
| Monoclonal | |
| 0.2 mg/mL | |
| PBS with 0.5% BSA, 10% proprietary stabilizer and 0.05% sodium azide; pH 7.2 | |
| P08670 | |
| Mouse | |
| 100 μg | |
| Primary | |
| Human | |
| Antibody | |
| IgG1 κ |
| Immunohistochemistry (Paraffin) | |
| V9 | |
| Alexa Fluor 420 | |
| Vim | |
| cb28; class-III intermediate microfilament; CTRCT30; epididymis luminal protein 113; FLJ36605; HEL113; intermediate filament; intermediate filament protein; RP11-124N14.1; similar to vimentin; VIM; VIME; Vimentin; vimentin C-terminus | |
| Affinity chromatography | |
| RUO | |
| 7431 | |
| 4° C, store in dark | |
| Liquid |