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Cytiva Thermo Sequenase DNA Polymerase
Novel thermostable DNA polymerase that uses dideoxynucleotide triphosphates (ddNTPs) as readily as deoxynucleotide (dNTP) substrates
Supplier: Cytiva E79000Z
Description
- Uniform and easy-to-read band patterns
- Better incorporation of ddNTPs helps avoid secondary structures that can cause unreadable sequences
- Read-through of difficult sequences (e.g., GC-rich), due to the ability of thermostable enzymes to function at elevated temperature used for sequencing
- ddNTPs are chain-elongating inhibitors of DNA polymerase, which are used in the Sanger method for DNA sequencing
- Each nucleotide base of this particular type has a probability of being bonded not to a deoxynucleotide but rather a dideoxynucleotide, which ends chain elongation
- An excellent choice for cycle sequencing where template quantity limitations preclude the use of T7 Sequenase DNA polymerase
- Can also be used in many SNP genotyping, genotyping by sequencing, or genome sequencing applications where primer extension is employed
Spécifications
| -20°C, Solution in 20 mM Tris-HCl, pH 8.5, 0.1 mM EDTA, 0.5% Tween 20 (v/v), 0.5% Nonidet P-40 (v/v), 1 mM DTT, 100 mM KCl, 50% glycerol | |
| 10,000 Units | |
| 32 units/μL | |
| E. coli clones that overproduce the protein |
| Thermo Sequenase DNA Polymerase | |
| Sequencing, Genotyping | |
| Thermo Sequenase/TAP, Dilution Buffer, and Reaction Buffer |