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Thermo Scientific™ B-PER™ Complete Bacterial Protein Extraction Reagent
Efficient, gentle lysis and extraction of E.coli and other bacterial cells
Supplier: Thermo Scientific™ 89821
Includes: Reagent packages are available in Tris and phosphate buffer formulations; Kits contain Tris-based B-PER Reagent plus lysozyme and DNase I enzymes.
Description
- An easy-to-use cell lysis reagent that effectively disrupts cells and solubilizes native or recombinant proteins without denaturation
- All B-PER Reagents are compatible with downstream applications such as affinity chromatography (e.g., immobilized metal affinity chromatography, glutathione chromatography), SDS-PAGE, and protein quantification (e.g., Pierce BCA Protein Assay, Pierce 660nm Protein Assay)
- This all-inclusive formulation combines the lysis reagent with lysozyme, and a universal nuclease to enable mild extraction from both gram-negative and gram-positive bacteria
- Depending on the particular application protease inhibitors, salts, reducing agents, denaturants, and chelating agents may be added to the reagent.
Features of the B-PER Bacterial Protein Extraction Reagent:
- Detergent in Tris buffer with Lysozyme and Universal Nuclease
- Improved cell lysis and DNA digestion, thereby releasing soluble proteins and reducing viscosity to increase yields
- Recovery of large molecular weight proteins (high MW proteins)
- Cost-effective equivalent to Merck Millipore Bugbuster™ Master Mix
- More effective than traditional sonication and typical homemade lysis buffers, many of which include detergents and components that interfere with downstream applications
Specifications
| Proprietary detergent in 20mM Tris buffer, pH 7.5, containing lysozyme and universal nuclease | |
| Bacterial Protein Extraction Reagent | |
| Store at 4°C. |
| 250 mL | |
| 250 mL | |
| B-PER™ |
For Research Use Only. Not for use in diagnostic procedures.