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MilliporeSigma™ Stem Cell Qualified ECM Gel

Stem cell qualified basement membrane extract (BME) for human ES and iPS cell culture.

Supplier: MilliporeSigma™ CC1315ML10P

Catalog No. CC1315ML10P

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Description

Stem Cell Qualified ECM Gel is a soluble basement membrane extract (BME) purified from Engelbreth-Holm-Swarm (EHS) tumor that has been pre-qualified for human ES/iPS cell culture
The matrix polymerizes at 20 to 40°C to form a reconstituted basement membrane
ECM Gel contains laminin, collagen IV, entactin and heparan sulfate among other ECMs
The matrix has been extensively tested and optimized for human ES/iPS cell culture
Eliminates the need for time-consuming prescreening and lot identification
Provides an optimized ECM coating necessary for long term culture of pluripotent human ES/iPS cells
Supports feeder-free culture of human ES/iPS cells in serum-free media
Compatible with all feeder free human ES/iPS cell expansion media (i.e. mTeSR™, PluriSTEM™)

Specifications

Form	Gel
Quantity	10 x 5 mL
Endotoxin Level	< 20 EU/mL
Content And Storage	Store at -20°C, thaw overnight on ice in a 2 to 8°C refrigerator

For Use With (Application)	Human ES and iPS cell culture
Assays	< 5% spontaneous differentiation for 3 passages using Human iPS cells
Color	Red, clear of particulates
Description	Basement membrane extract (BME)

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