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Cytiva RNAspin 96 RNA Isolation Kit
For fast and efficient purification in a 96-well format
Supplier: Cytiva 25050075
Includes: Protocol and sufficient quantities of the following to carry out the number of preps indicated: DNase 1, RNase-free water, binding plates, 1.5mL tubes, wash plates, square well blocks, vacuum elution plates and buffers
Description
- 96 samples parallel-processed via either centrifugation or vacuum with high reproducibility in under 70 minutes
- Can be used to isolate total RNA from cultured cells, tissue, bacteria or yeast
- DNase I treatment removes genomic DNA resulting in high-quality total RNA product
- Isolated RNA is of sufficient quality (A260/A280 = 1.8-2.2) and quantity (up to 100μg) for multiple downstream experiments: quantitative reverse transcription polymerase chain reaction (QRT-PCR), primer extension and RNase protection assays
- Integrated wash plate eliminates risk of contamination
- Accessory tissue plate allows tissue samples to be processed
- Sample size: up to 30mg for animal tissue, up to 2 x 106 cells for cell culture (vaccum); 1x 107 cells for cell culture (centrifuge); Elution volume: 50μ-130μL
Specifications
| illustra RNAspin 96 Kit | |
| Used to reproducibly (well-to-well, plate-to-plate) isolate total RNA from cultured cells, tissue, bacteria or yeastCan also be used in a fully automated flow with vacuum on common laboratory workstationsSuitable for direct use in downstream application | |
| 4 x 96 Preps | |
| 1.8-2.2 | |
| 70 min/4 preps |
| 96-well Plate | |
| Protocol and sufficient quantities of the following to carry out the number of preps indicated, DNase 1, RNase-free Water, binding plates, 1.5mL tubes, wash plates, square well blocks, vacuum elution plates and Buffers | |
| illustra RNAspin 96 Kit | |
| 10 mg tissue-30 mg tissue |