Invitrogen™ Rat anti-Mouse IgG2a Secondary Antibody, HRP

Description

Mouse gamma 2a heavy chain of immunoglobulin (determined by immunodot). No crossreactivity with other animals. Useful in immunoassays, such as ELISA's (good binding on plastic), immunohistochemistry on frozen sections and for affinity chromatog¬raphy of murine IgG1. Available unconjugated MON5068, HRP conjugated 5068H.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.