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Invitrogen™ PureLink™ Expi Endotoxin-Free Buffer Set 
Specifically, the buffer set includes RNAse A, Resuspension Buffer (R3), Lysis Buffer (L7), Precipitation Buffer (N3), Equilibration Buffer (EQ1), Wash Buffer (W8), Elution buffer (E4), TE Buffer (TE), Endotoxin Removal Buffer, Lysis Indicator, and endotoxin free water.
Supplier: Invitrogen™ A33074
Description
Specifically, the buffer set includes RNAse A, Resuspension Buffer (R3), Lysis Buffer (L7), Precipitation Buffer (N3), Equilibration Buffer (EQ1), Wash Buffer (W8), Elution buffer (E4), TE Buffer (TE), Endotoxin Removal Buffer, Lysis Indicator, and endotoxin free water.
- Simple and super-fast protocol—Unlike other anion-exchange-based endotoxin free plasmid purification systems, the PureLink™ Expi Endotoxin-Free Maxi Plasmid Purification Kit utilizes vacuum filtration for lysate clarification and plasmid DNA purification. There is no waiting for gravity-flow columns and typical purification takes 90 minutes.
- High yield—solate up to 1.5 mg of high quality, endotoxin-free plasmid DNA
- Endotoxin-Free—Purify endotoxin-free (<0.1 EU/ μg) plasmid that is ideal for sensitive applications such as transfection of primary cells, genome engineering, and research on gene therapy or plasmid vaccines.
- Purity—Ultralow levels of protein, RNA and genomic DNA contamination
- Compatibility with ExpiCHO™ and Expi293™ Expression Systems—Plasmid purified using the PureLink™ Expi Endotoxin-Free Plasmid Purification Kit is compatible with the Gibco™ ExpiCHO™ and Expi293™ transient mammalian expression systems.
Specifications
| • Resuspension buffer • Lysis indicator • RNase A • Lysis buffer • Precipitation buffer • Endotoxin removal buffer • Wash buffer • Elution buffer • TE buffer |
|
| Bacteria | |
| Cloning, PCR | |
| > 1 mg (Large-Scale) Plasmid DNA, > 200 μg (Large-Scale) Plasmid DNA | |
| Buffer Set | |
| Room Temperature | |
| 90 min. |
| Maxi | |
| Mammalian | |
| Manual Protocols | |
| PureLink™ | |
| 1 Set | |
| Plasmid DNA |
For Research Use Only. Not for use in diagnostic procedures.