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MilliporeSigma™ ProteoExtract™ Native Cytoskeleton Enrichment
This method provides means to directly study cytoskeleton-associated pool of proteins in normal and diseased cytoskeletons
Supplier: MilliporeSigma™ 1710210
Includes:
- One bottle containing 1.9mL of 10x Cellular Extraction Buffer
- One bottle containing 3.5mL of 20x Cytoskeleton Wash Buffer
- One bottle containing 9mL of ready to use Nuclear Extraction Buffer
- One vial containing 100μL of 1000x Protease Inhibitor Cocktail solution
- One vial containing 150μL of 1000x Sodium Orthovanadate solution
- One vial containing 50μL of 200x donkey anti-mouse FITC conjugate
- One bottle containing 12.5mL of 10x Blocking/Permeabilization Buffer
- One vial containing 15μg lyophilized TRITC-Conjugated Phalloidin
- One vial containing 100μL of 200x DAPI
- One vial containing 100μL of 100x anti-Vinculin mouse monoclonal IgG1 antibody
- One vial containing 16μL of 500x anti-GAPDH mouse monoclonal IgG1 antibody
Requires :
- Pipettors and tips capable of accurately measuring 1-1000μL
- Graduated serological pipettes
- Distilled or deionized water
- 4% paraformaldehyde solution for fixing cells
- 1x Dulbecco's Phosphate Buffered Saline (D-PBS)
- Cover slips
- Mounting fluid
Description
- Provides cytoskeleton purification detergent buffers, sufficient for extraction from ten 100mm culture dishes to retain focal adhesion and actin-associated proteins while removing soluble cytoplasmic and nuclear proteins from the cell.
- Anti-Vimentin and anti-GAPDH antibodies are provided as markers for cytoskeleton and cytosol, respectively, in western blot analysis
- This kit will greatly increase the ability to detect and study the low abundance actin-associated proteins which are typically masked in conventional methods of Western blotting analysis of whole cell lysates
Specifications
| The kit may be used for up to 4 months after receipt. Avoid repeated freeze/thaw cycles, and aliquot if necessary | |
| Immunofluorescence, Immunocytochemistry | |
| 32 Assays/Kit |