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MilliporeSigma™ Proteinase K, Lyophilized
Highly active serine protease that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA
$215.00 - $701.00
Specifications
| CAS | 39450-01-6 |
|---|---|
| Content And Storage | 2°C to 8°C |
| Product Type | Proteinase K |
Description
- Proteinase K is a highly active 28,904-Da serine protease isolated from the fungus Tritirachium album
- The enzyme exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA
- Its activity is increased in the presence of denaturants such as SDS (1%) and elevated temperature (50-60°C)
- The recommended working concentration is 50-100μg/mL for protein removal and enzyme inactivation, and up to 2mg/mL for tissue treatment
- The Proteinase K, Lyophilized powder can be prepared as a 20mg/mL stock solution in water and stored in aliquots at -20°C
- The enzyme is also available as a ready-to-use concentrated stock solution (600mAU/mL) that is convenient for routine use in most applications
- 1mg of Proteinase K is the equivalent of 30mAU (AU = Anson unit)
- The Novagen Proteinase K products are free of detectable DNase and RNase
Specifications
| 39450-01-6 | |
| 2°C to 8°C | |
| Proteinase K |