Thermo Scientific™ Stop Solution for TMB Substrates

Description

Thermo Scientific™ Pierce Stop Solution for TMB is 0.16M sulfuric acid for use with the ELISA substrate 3,3',5,5' - tetramethylbenzidine (TMB).

TMB Stop Solution is 0.16M sulfuric acid for use with the ELISA substrate 3,3«,5,5«-tetramethylbenzidine (TMB). Addition of sulfuric acid stop solution changes the color from blue to yellow, stabilizing the color development to enable accurate measurement of the intensity at 450nm using a spectrophotometer or plate reader.

• Size: 55mL

• Sufficient For: five 96-well microplates

• Application: ELISA

Instructions: Following TMB incubation, add an amount of stop solution equal to the amount of TMB substrate added to each well of the ELISA plate (typically 50 to 100μL per well). Use at full strength.