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MP Biomedicals™ 0.25% Trypsin-EDTA (1X)
0.25% Trypsin and 1mM EDTA in modified HBSS w/o Ca++ and Mg++, 100 mL
Supplier: MP Biomedicals™ 091689649
Description
- Trypsin-EDTA solutions are used to release adherent cells from culture surfaces.
- Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues.
- The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.
- Ethylenediaminetetracetic acid (EDTA), a chelating agent, is added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cellto-cell adhesion and obscure the peptide bonds on which trypsin acts.
Specifications
| -20°C | |
| Cell Culture Dissociation Reagent | |
| No Calcium, No Magnesium | |
| 7 to 7.3 | |
| Porcine (for Trypsin) |
| 1:250 | |
| Sterile | |
| 0.25% (w/v) Solution, 0.25% Trypsin | |
| 100 mL |
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use.