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Invitrogen™ ImaGene Green™ C12FDG lacZ Gene Expression Kit 
Substrates cleaved within cell for fluorescent product
Supplier: Invitrogen™ I2904
Description
The ImaGene Green™ lacZ Gene Expression Kit contains a fluorescein-based galactosidase substrate that has been covalently modified to include a 12-carbon lipophilic moiety, C12FDG. Once inside the cell, the substrates are cleaved by β-galactosidase, producing a fluorescent product that is well retained by the cells, probably by incorporation of the lipophilic tail within the cellular membrane. In addition to the C12FDG, the kits also include the broad-spectrum β-galactosidase inhibitor, PETG, and chloroquine diphosphate for inhibiting acidic hydrolysis of the substrate.
Beta-Galactosidase Assays, Cell Analysis, Cellular Imaging, Enzyme and Protein Activity Assays, Flow Cytometry, Immunofluorescence (IF), Immunofluorescence Staining and Detection, Proteins, Expression, Isolation and Analysis, Reporter Assays
Order Info
Shipping Condition: Room Temperature
Specifications
| β-Galactosidase Reporter Gene Assay System | |
| β-Gal (lacZ) Assay | |
| Room Temperature | |
| Beta-Gal Substrate | |
| Beta-Galactosidase | |
| ImaGene Green |
| Fluorescence | |
| Store in freezer (-5 to -30°C) and protect from light. | |
| C12FDG | |
| Chemical Substrate | |
| Kit | |
| 1 kit |
For Research Use Only. Not for use in diagnostic procedures.