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Molecular Probes™ MitoProbe™ Transition Pore Assay Kit, for flow cytometry DFS Item

MitoProbe™ Transition Pore Assay Kit, for flow cytometry

Supplier:  Molecular Probes™ M34153

Catalog No. 501121533


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Description

Description

The MitoProbe™ Transition Pore Assay Kit provides a proven method (Petronilli V. et al. 1989, 1999) of measuring mitochondrial permeability transition pore opening. The kit includes a dye and a quencher that are easily loaded into cells. In healthy cells, the dye, but not the quencher, enters mitochondria. The mitochondria remain brightly fluorescent until mitochondrial pore activation permits quenching of the fluorescence.

View a selection guide for all apoptosis assays for flow cytometry.
Specifications

Specifications

1 kit
Transition Pore Assay Kit
488
Room Temperature
Calcein AM
Tube
Contains 5 vials of calcein, AM (50 μg of dry powder/vial), 1 vial of cobalt (II) Chloride hexahydrate (1.2 mL, 80 mM in saline), 1 vial of ionomycin (55 μg), calcium salt, and 1 vial of DMSO (1.5 mL).

Store in freezer (-5 to -30°C).
100 Reactions
MitoProbe™
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For Research Use Only. Not for use in diagnostic procedures.