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Gibco™ McCoy's 5A (Modified) Medium, GlutaMAX™ Supplement
McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues.
Supplier: Gibco™ 36600021*
Description
McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.
This McCoy's 5A is modified as follows:
With: High Glucose, GlutaMAX™, Bacto-peptone, Phenol Red
Without: Sodium Pyruvate
The complete formulation is available.
Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO2 incubator.
Gibco™ McCoy's 5A (modified) requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). Gibco™ McCoy's 5A (modified) uses a sodium bicarbonate buffer system (2.2 g / L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Specifications
| Mammalian Cells | |
| 1 X | |
| McCoy's 5A Medium | |
| High Glucose, GlutaMAX, Phenol Red, Bacto-peptone, Sodium Bicarbonate | |
| cGMP-compliant under the ISO 13485 standard | |
| 500 mL | |
| 280 - 320 mOsm/kg | |
| Storage conditions: 2-8° C. Protect from light. Shipping conditions: Ambient. Shelf life: 12 months from date of manufacture. |
|
| Mammalian Cell Culture |
| Animal Origin-free | |
| Liquid | |
| Sterile-filtered | |
| No HEPES, No Sodium Pyruvate | |
| Gibco™, GlutaMAX™ | |
| Room Temperature | |
| 6.9 to 7.1 | |
| CO2 |