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Invitrogen™ Topoisomerase I

Catalyzes removal of superhelical turns from covalently closed DNA by a transient breakage and rejoining of phosphodiester bonds

Supplier: Invitrogen™ 38042024

Catalog No. 38042024

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Description

Topoisomerase I is active in the presence of EDTA
Source: Purified from calf thymus
Performance and Quality Testing: Endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease, and phosphatase assays; conversion of super-coiled DNA to relaxed DNA
Unit Definition: One unit catalyzes the conversion of 0.5μg of superhelical ΦX174 RF DNA to a relaxed state in 30 min. at 37°C
Unit Reaction Conditions: 50mM Tris-HCl (pH 7.5), 50mM KCl, 10mM MgCl₂ , 0.1mM EDTA, 0.5mM DTT, 30 μg/mL BSA, 0.5μg ΦX174 RF DNA, and enzyme in 50μL for 30 min. at 37°C

Relaxing positively and negatively supercoiled DNA, Producing DNA topoisomers, Cloning, Restriction Enzyme Cloning

Shipping Conditions: Wet ice

Specifications

Shipping Condition	Wet Ice
Quantity	500 U

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For Research Use Only. Not for use in diagnostic procedures.

Description