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Invitrogen™ Platinum™ PCR SuperMix High Fidelity 
Platinum PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.
$578.00 - $23720.00
Specifications
| GC-Rich PCR Performance | Low |
|---|---|
| Polymerase | Platinum Taq DNA Polymerase High Fidelity |
| Reaction Speed | Standard |
| Shipping Condition | Wet or Dry Ice |
| For Use With (Application) | Hot-start PCR, High-fidelity PCR |
Includes: Platinum PCR SuperMix High Fidelity is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 100 amplification reactions of 50μL each are provided (4 x 1.125mL). Platinum PCR SuperMix High Fidelity Components: 22U/mL complexed recombinant Taq DNA polymerase, Pyrococcus species GB-D thermostable polymerase, and Platinum Taq Antibody; 66mM Tris-SO4 (pH 8.9); 19.8mM (NH4)2SO4; 2.4mM MgSO4; 220μM dNTPs; and stabilizers.
| Catalog Number | Mfr. No. | No. of Reactions | Price | Quantity | |||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | No. of Reactions | Price | Quantity | |||||
|
12532016
|
Invitrogen™
12532016 |
100 Reactions |
Each for $578.00
|
|
|||||
|
12532024
|
Invitrogen™
12532024 |
5000 Reactions |
N/A
|
N/A | |||||
| Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance. | |||||||||
Description
Platinum PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Designed for high-specificity, high-fidelity DNA amplification, Platinum PCR SuperMix reduces reaction set-up time and only requires the addition of template and primer.
High fidelity is provided by a mixture of Taq DNA polymerase and the proofreading Pyrococcus species GB-D polymerase. High specificity is achieved by anti-Taq antibodies, allowing for automatic hot-start PCR.
Benefits of Platinum PCR SuperMix, High Fidelity
- Six-times higher fidelity than Taq DNA polymerase
- Amplifies fragments up to 15 kb
- Minimizes PCR optimization
- Room-temperature reaction assembly
Applications
- High-fidelity PCR
- Cloning
- Mutagenesis
Notes
- Platinum PCR SuperMix, High Fidelity, produces products that are mixed blunt and 3-A' ends; however, the majority of ends will have a 3-A' overhangs.
- Hot-start capability increases specificity and yield and allows for room-temperature reaction assembly.
Order Info
Approved for shipment on Wet or Dry Ice
Specifications
| Low | |
| Standard | |
| Hot-start PCR, High-fidelity PCR | |
| 6X | |
| Mixed | |
| 15 kb or less |
| Platinum Taq DNA Polymerase High Fidelity | |
| Wet or Dry Ice | |
| 1.1X | |
| Built-In Hot Start | |
| SuperMix or Master Mix |
For Research Use Only. Not for use in diagnostic procedures.