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Invitrogen™ PCRX Enhancer System 
PCRx Enhancer System is used in conjunction with DNA polymerases to optimize PCR of problematic and/or GC-rich templates.
Supplier: Invitrogen™ 11495017
Includes: PCRx Enhancer Solution consists of 10X PCRx Amplification Buffer, 10X PCRx Enhancer Solution, and 50mM magnesium sulfate.
Description
PCRX Enhancer System is an optimized buffer and cosolvent system designed for use in conjunction with DNA polymerases to optimize PCR of problematic and/or GC-rich templates. 10X PCRX Enhancer Solution with 10X PCRX Amplification Buffer offers higher primer specificity, broader magnesium concentration, broader annealing temperature, and improved thermostability of DNA polymerases such as Taq DNA polymerase and Platinum Taq DNA Polymerase.
Conventional PCR buffer components can be directly substituted with the 10X PCRX amplification buffer and 50 mM MgSO4 for a more robust amplification and improved product yield in some cases.
Notes
Optimal concentration of 10X PCRX Enhancer Solution will vary depending on GC content, Mg++ concentration, and annealing temperature.
Order Info
Shipping Condition: Dry Ice
Specifications
| • 10X PCRx Enhancer Solution (2 x 1 mL) • 10X PCRx Amplification Buffer (2 x 1 mL) • 50 mM MgSO4 (1 x 1 mL) Store at -20°C. |
|
| 250 rxns | |
| Standard PCR, Hot-start PCR | |
| Separate Components |
| Platinum Taq DNA Polymerase, Platinum Taq DNA Polymerase High Fidelity, Taq DNA Polymerase | |
| Dry Ice | |
| 250 Reactions | |
| DNA |
For Research Use Only. Not for use in diagnostic procedures.