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Invitrogen™ Gateway™ pET-DEST42 Vector 
Designed for rapid cloning with Gateway entry clone using lambda phage site-specific recombination and subsequent high-level prokaryotic expression controlled by strong bacteriophage T7 promoter
Supplier: Invitrogen™ 12276010
Includes: The Champion pET-DEST42 Gateway destination vector includes 6μg of vector.
Description
- Expression is induced by production of T7 RNA polymerase in BL21(DE3) E. coli
- Bacteriophage T7lac promoter for high-level expression
- O operator sequences for lac repressor binding to ensure tighter regulation of transcription
- pBR322 ori for minimal basal expression
- C-terminal V5 and 6xHis tags for efficient detection and purification
- R sites for efficient recombination with any attL-flanked Gateway entry vector
Bacterial Expression, Bioproduction, Cloning, Gateway Cloning, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
| Inducible | |
| T7, lacO | |
| None | |
| Ampicillin (AmpR) | |
| Gateway | |
| pET, pDEST |
| IPTG | |
| Gateway System Destination Expression Vector | |
| The Champion™ pET-DEST42 Gateway™ destination vector includes 6 μg of vector. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored. | |
| His Tag (6x), V5 Epitope Tag | |
| 6 μg | |
| Gateway™ |
For Research Use Only. Not for use in diagnostic procedures.