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Invitrogen™ pcDNA™6.2-DEST Mammalian Expression Vector DFS Item

Designed for high-level, constitutive expression in variety of mammalian cell lines

Supplier:  Invitrogen™ 12489027

Catalog No. 12489027


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Includes: All destination vectors are provided lyophilized and supercoiled.

Requires : Gateway entry clone, Gateway LR Clonase™ enzyme mix, and reaction buffer.

Description

Description

  • Cytomegalovirus (CMV) promoter for high-level expression
  • attR sites for Gateway cloning, enabling recombination with attL-flanked fragments
  • C-terminal V5 tag for easy detection
  • Neomycin resistance gene for stable selection
  • Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
  • Gateway Cloning
  • Destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an UltimateORF Clone

Cloning,Constitutive Expression,Gateway Cloning,Mammalian Expression,Protein Expression,Proteins, Expression, Isolation and Analysis

Specifications

Specifications

Constitutive
CMV
Blasticidin
V5 Epitope Tag
6 μg
Gateway™, pcDNA™
Transfection
Mammalian Expression Vector
All destination vectors are provided lyophilized and supercoiled.
Gateway
pDEST, pcDNA
Constitutive Expression
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For Research Use Only. Not for use in diagnostic procedures.