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Invitrogen™ β-Gal Staining Kit 
Determine percentage of transfected cells expressing lacZ
Supplier: Invitrogen™ K146501
Includes: Sufficient reagents to stain fifty 60mM plates. The kit includes 10X PBS (phosphate buffered saline), X-gal, staining solutions A, B, and C, 10X fixative solution, and 10μg of pcDNA 3.1/His/lacZ control vector.
Description
- lacZ is bacterial gene often used as reporter construct in eukaryotic transfection experiments
- Gene product of lacZ, β-galactosidase, is resistant to proteolysis in cellular lysates, and its activity is easily assayed
- β-galactosidase catalyzes hydrolysis of X-gal, producing a blue color that can be easily visualized under microscope
- Colorimetric detection
Beta-Galactosidase Assays/Enzyme and Protein Activity Assays/Proteins, Expression, Isolation and Analysis/Reporter Assays
Specifications
| The β-Gal Staining Kit contains sufficient reagents to stain fifty 60 mm plates. The kit includes 10X PBS (phosphate buffered saline), X-gal, staining solutions A, B, and C, 10X fixative solution, and 10 μg of pcDNA™ 3.1/His/lacZ control vector. Store the 10X PBS at room temperature. All other components should be stored at -20°C. All reagents are guaranteed stable for 6 months when properly stored. | |
| Colorimetric | |
| X-Gal | |
| Beta-Galactosidase |
| β-Gal (lacZ) Assay | |
| Beta-Gal Staining Kit | |
| 1 Kit |
For Research Use Only. Not for use in diagnostic procedures.