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Invitrogen™ β-Gal Assay Kit

Provides reagents required to quickly measure levels of active β-galactosidase expressed in cells transfected with plasmids expressing lacZgene

Supplier: Invitrogen™ K145501

Catalog No. K145501

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Quantity

Includes: 10X PBS (phosphate buffered saline), lysis buffer, cleavage buffer, β-mercaptoethanol, ONPG, stop buffer.

Description

lacZis bacterial gene often used as reporter construct in eukaryotic transfection experiments
Gene product of lacZ, β-galactosidase, is resistant to proteolysis in cellular lysates, and its activity is easily assayed
β-galactosidase catalyzes hydrolysis of β-galactosidase such as ortho-nitrophenyl-D-galactopyranoside (ONPG)
Hydrolysis of ONPG to ONP anion produces bright yellow color with peak absorbance at 420nm that can be quantified using spectrophotometer
10.7 gene expression
Colorimetric detection

Beta-Galactosidase Assays, Enzyme and Protein Activity Assays, Proteins, Expression, Isolation and Analysis, Reporter Assays

Specifications

Content And Storage	The β-Gal Assay Kit contains 10X PBS (phosphate buffered saline), lysis buffer, cleavage buffer, β-mercaptoethanol, ONPG, stop buffer. Store ONPG at -20°C. All other components are stored at either room temperature or +4°C. All reagents are guaranteed stable for 6 months when properly stored.
Detection Method	Colorimetric
Substrate	ONPG
Target	Beta-Galactosidase

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For Research Use Only. Not for use in diagnostic procedures.

Description