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Invitrogen™ ElectroMAX™ DH10B T1 Phage-Resistant Competent Cells 
ElectroMAX DH10B T1 Phage-Resistant Competent Cells offer transformation efficiencies that are among the highest we offer and can exceed 1 x 1010 cfu/μg plasmid DNA in efficiency.
Supplier: Invitrogen™ 12033015
Includes: ElectroMAX DH10B T1 Phage-Resistant Cells: 5 vials, 100μL each, pUC19 DNA (10pg/μL): 1 vial, 50μL, S.O.C. medium: 2 bottles, 6mL each
Description
ElectroMAX DH10B T1 Phage-Resistant Competent Cells offer transformation efficiencies that are among the highest we offer and can exceed 1 x 1010 cfu/μg plasmid DNA in efficiency. They are ideal for applications requiring high-efficiency transformation, including cDNA and gDNA library construction.
ElectroMAX DH10B cells:
• Maximize yield of transformants from limited cloning products
• Permit efficient cloning of methylated DNA
• Are resistant to T1 and T5 bacteriophages
• Support blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal
• Deliver high-yield plasmid preparations for downstream applications
Phage-resistant representative libraries from a single transformation
The tonA mutation in DH10B cells confers resistance to bacteriophages T1 and T5, safeguarding valuable libraries against contamination. Additionally, mutations in the methylation-dependent restriction system (mcrA, mcrBC, and mrr) make ElectroMAX DH10B T1 Phage-Resistant Cells ideal for construction of genomic libraries of both prokaryotic and eukaryotic genomic DNA and allow for efficient plasmid rescue from eukaryotic genomes. ElectroMAX DH10B T1 Phage-Resistant Cells are suitable for construction of gene banks, for the generation of cDNA libraries using plasmid-derived vectors, and for situations when DNA is limiting. This strain also has the Φ80lacZΔM15 genotype, providing for the option of blue/white screening on plates containing either X-Gal or Bluo-Gal. Finally, the endA1 mutation makes DH10B an excellent vehicle for amplifying plasmid DNA for subsequent extraction and purification.
Genotype
F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ(ara, leu)7697 galU galK λ-rpsL nupG tonA
Find the strain and format that fits your needs
• DH10B cells are available in both electrocompetent and chemically competent formats.
• ElectroMAX DH10B cells (Cat. No.18290015) are suitable for cDNA or gDNA library construction but are not resistant to T1 and T5 phage infection.
• MegaX DH10B T1R Electrocomp Cells (Cat. No. C640003) have the highest transformation efficiency (>3 x 1010 cfu/μg plasmid DNA) and also offer the benefit of T1 and T5 phage resistance.
Order Info
Shipping Condition: Dry Ice
Specifications
| Electrocompetent Cells | |
| Yes (endA1) | |
| High Efficiency (>1 x 109 cfu/μg) | |
| Yes (Streptomycin) | |
| Yes (lacZΔM15) | |
| May be used for plasmids >20 kb | |
| Yes (recA1) | |
| Yes | |
| Tube | |
| 5 x 100 μL |
| No | |
| Yes (mcrA) | |
| • ElectroMAX DH10B T1 Phage-Resistant Cells (5 x 100 μL) Store Competent Cells at –80°C. • pUC19 DNA (50 μL at 10 pg/μL) Store pUC19 DNA at –20°C. • S.O.C. Medium (2 x 6 mL) Store S.O.C. Medium at 4°C or room temperature. . |
|
| Not suitable for cloning unstable DNA | |
| Low | |
| No | |
| Dry Ice | |
| E. coli (K12) | |
| ElectroMAX™ |
For Research Use Only. Not for use in diagnostic procedures.