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Invitrogen™ Biotin-14-dCTP 
Used for DNA biotinylation, an excellent, and sometimes superior alternative to conventional radioisotopic labeling
Supplier: Invitrogen™ 19518018
Includes: Biotin-14-dCTP is supplied at 0.4mM in 100mM Tris-HCl (pH 7.5), 0.1mM EDTA.
Description
About Biotin-14-dCTP
Biotin-14-dCTP is a patented dCTP analog with biotin attached at the N4-position of the pyrimidine base by a 14-atom linker. This nucleotide can be efficiently incorporated into DNA by nick translation in the presence of dATP, dGTP, and dTTP, labeling DNA with terminal deoxynucleotide transferase, or random primer synthesis. The amount of material provided is sufficient for labeling up to 50μg of DNA.
Performance and quality testing
Purity is evaluated by reverse-phase HPLC.
- Convenience and safety: probe preparation generates no radioactive waste
- Stability: probes remain active longer than isotopically labeled probes
- Versatility: biotinylated DNA can be used for immobilization and capture procedures via streptavidin binding
DNA and RNA Purification and Analysis, DNA Labeling, Nucleic Acid Labeling and Oligo Synthesis
Order Info
Shipping Condition: Dry Ice
Specifications
| Labeled Nucleotide | |
| Biotin-14-dCTP is supplied at 0.4 mM in 100 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Store at -20°C. | |
| Direct Labeling | |
| 50 nmol |
| 0.4 mM | |
| Biotin & Other Hapten | |
| Dry Ice | |
| Liquid |
For Research Use Only. Not for use in diagnostic procedures.