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Invitrogen™ BaculoDirect™ C-Term Expression Kit

Have attR sites for recombination with any attL-flanked fragment, regardless of whether it is entry clone or Ultimate™ RF Clone

Supplier: Invitrogen™ 12562013

Catalog No. 12562013

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Quantity

Includes: All destination vectors are provided lyophilized and supercoiled.

Requires : Gateway™ entry clone, Gateway™ LR Clonase enzyme mix, and reaction buffer.

Description

Baculovirus Production and Titering, Cloning, Gateway Cloning, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

Promoter	Polyhedrin
Content And Storage	The BaculoDirect expression kits include five 0.3 Âµg vials of BaculoDirect Linear DNA, 125 ÂµL of Cellfectin transfection reagent, frozen Sf9 cells, Gateway LR Clonase enzyme mix, GIBCO Unsupplemented Grace's Insect Medium, and ganciclovir. Store the BaculoDirect Linear DNA, Cellfectin reagent, and Grace's Medium at +4°C. Store LR Clonase mix at -80°C. Store Sf9 cells in liquid nitrogen. All components are guaranteed stable for 6 months if stored properly.
Cloning Method	Gateway
Vector	BaculoDirect Vectors

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For Research Use Only. Not for use in diagnostic procedures.

Description