Invitrogen™ AccuPrime™ Taq DNA Polymerase System 

Description

The AccuPrime Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases.

Platinum anti-Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR. This combination improves the fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR. AccuPrime Taq DNA Polymerase broadens primer annealing temperatures, giving you optimal performance between 55°C and 65°C.

AccuPrime Taq DNA Polymerase features

• Control mispriming at every cycle for single-band PCR products
• Minimal reaction optimization and primer set redesign
• Reaction assembly at room temperature

Applications

AccuPrime Taq DNA Polymerase can be used for a wide variety of applications, including:

• Multiplex PCR
• TOPO TA Cloning
• Allele-specific amplifications
• High-throughput screening

AccuPrime buffers

The 10X AccuPrime buffers contain thermostable AccuPrime protein, Mg⁺⁺, and deoxyribonucleotide triphosphates at concentrations sufficient to allow amplification during PCR. Two individual buffer systems (10X AccuPrime PCR Buffer I and II) are provided for amplification of specific types of templates. 10X AccuPrime PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids. 10X AccuPrime PCR Buffer II is designed for genomic DNA (200 bp–4 kb).