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Invitrogen™ AccuPrime™ Pfx SuperMix DFS Item

AccuPrime Pfx SuperMix is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification.

Supplier:  Invitrogen™ 12344040

Catalog No. 12344040


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Includes: AccuPrime Pfx SuperMix is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 200 amplification reactions of 25μL each (4 x 1.125mL) are provided. AccuPrime Pfx SuperMix Components: 22U/mL Thermococcus species KOD thermostable polymerase complexed with anti-KOD antibodies, 66mM Tris-SO4 (pH 8.4), 30.8mM (NH4)2SO4, 11mM KCl, 1.1mM MgSO4, 330μM dNTPs, AccuPrime proteins, stabilizers.

Requires : Template and primers

Description

Description

Invitrogen AccuPrime Pfx SuperMix provides reagents for high-fidelity, high-specificity amplification of DNA fragments. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading activity. This ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs is designed for efficient PCR amplification.

Features

  • >26-times higher fidelity than with Taq DNA polymerase
  • Reduced set-up time
  • Produces products with blunt ends

Applications

  • High-fidelity PCR
  • Amplification of fragments up to 15 kb
  • Cloning & mutagenesis

Notes

  • Thermostable AccuPrime Pfx SuperMix proteins enhance primer-template hybridization during every cycle, increasing specificity and yield.

Order Info

Approved for shipment on Wet or Dry Ice

Specifications

Specifications

1.1X
Primer-probe
AccuPrime Pfx DNA Polymerase
200 rxns
Hot-start PCR, High-fidelity PCR
Built-In Hot Start
Blunt
15 kb or less
AccuPrime Pfx SuperMix (4 × 1.125 mL)

Store at –20°C in a non-frost-free freezer.
Low
Standard
Wet or Dry Ice
26X
200 Reactions
SuperMix or Master Mix
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For Research Use Only. Not for use in diagnostic procedures.