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Cytiva IMAC Sepharose™ 6 Fast Flow Affinity Media
Used for purifying proteins and peptides that have an affinity for metal ions. Cytiva IMAC Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix.
Supplier: Cytiva 17092108
Description
- Uncharged IMAC resin allows possibility to charge with metal ion of choice for optimized selectivity
- Used for ions such as histidine-tagged proteins but also untagged recombinant or native proteins
- High protein binding capacity
- Coupling chemistry and novel ligand designed to give minimal ligand leakage
- Available HiTrap™ and HiPrep™ pre-packed formats for easy scale-up and process development
Specifications
| 100 mL | |
| Stable in commonly used aqueous Buffers: 0.01 M HCl, 1 M NaOH, 70% Acetic Acid, 2% SDS, 30% 2-Propanol | |
| IMAC Sepharose 6 Fast Flow Media | |
| 45 to 165 μm | |
| 25 cm | |
| 6% Cross-Linked Agarose | |
| 20% Ethanol |
| For Purifying Proteins and Peptides That Have an Affinity for Metal Ions | |
| 4°C to 30°C | |
| 250 to 400 cm/Hoμr | |
| 9.84 in. | |
| 100 kPa | |
| 3 to 12 |