IL-7 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

AA Sequence of recombinant protein: MDCHIKDKDG KAFGSVLMIS INQLDKMTGT DSDCPNNEPN FFKKHLCDDT KEAAFLNRAA RKLRQFLKMN ISEEFNDHLL RVSDGTQTLV NCTSKEEKTI KEQKKNDPCF LKRLLREIKT CWNKILKGSI. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat IL-7. Anti-Rat IL-7-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Rat IL-7 by sandwich ELISA (using 100 mL/well) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat IL-7 (500-P310) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-7. Western Blot: To detect Rat IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 mg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Rat IL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P310BT-1MG will be provided as 2 x 500 μg

Interleukin 7 (IL-7) is a hematopoietic cytokine that is constitutively produced by stromal cells from the bone marrow and thymus. IL-7 signals through the IL-7R to promote the differentiation of hematopoietic stem cells to lymphoid precursor cells, which give rise to T cells, B cells, and natural killer cells.