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Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement, HEPES 
Originally developed to culture human leukemic cells in suspension and as a monolayer
Supplier: Gibco™ 72400146
Description
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 medium) has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.
- Modified as follows: with GlutaMAX™, phenol red, HEPES
- Contains reducing agent glutathione and high concentrations of vitamins
- Contains biotin, vitamin B12 and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
- Vitamins inositol and choline are present in very high concentrations
- GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format
- Contains no proteins, lipids, or growth factors
- Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
- Uses sodium bicarbonate buffer system (2.0g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH
Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Cell Culture
Specifications
| HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas | |
| Animal Origin-free | |
| Liquid | |
| Standard Serum Supplementation | |
| GlutaMAX, HEPES, Phenol Red | |
| Gibco™, GlutaMAX™, SynerGy™ | |
| Room Temperature |
| Leukemic Cells | |
| 1 X | |
| RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) | |
| Sterile-filtered | |
| No Sodium Pyruvate | |
| 5 L | |
| Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.