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Gibco™ MEM α, nucleosides 
MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells.
Supplier: Gibco™ 12571048
Description
MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This MEM α is modified as follows:
With: Ribonucleosides, Deoxyribonucleosides, Phenol Red, L-glutamine
The complete formulation is available.
Using MEM α
MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This product is made with Earle's salts. MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Specifications
| HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts | |
| Animal Origin-free | |
| Liquid | |
| Standard Serum Supplementation | |
| Sterile-filtered | |
| No HEPES | |
| Gibco™ | |
| Room Temperature |
| Primary Rat Astrocytes | |
| 1 X | |
| MEM α (Minimum Essential Medium α) | |
| Sterile-filtered | |
| Low Glucose, Phenol Red, Sodium Pyruvate, Ribonucleosides, Deoxyribonucleosides | |
| cGMP-compliant under the ISO 13485 standard | |
| 1000 mL | |
| Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.