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Gibco™ MEM α, no nucleosides, powder 
Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells.
$79.50 - $121.00
Specifications
| Cell Line | HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts |
|---|---|
| Cell Type | Primary Rat Astrocytes |
| Classification | Animal Origin-free |
| Form | Powder |
| Product Type | MEM α (Minimum Essential Medium α) |
Description
Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco™ MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This MEM α is modified as follows:
With: Phenol Red, L-glutamine
Without: Ribonucleosides, Deoxyribonucleosides, Sodium Bicarbonate
The complete formulation is available.
Gibco™ MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. This product is made with Earle's salts.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
Order Info
Shipping Condition: Room Temperature
Specifications
| HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts | |
| Animal Origin-free | |
| MEM α (Minimum Essential Medium α) | |
| Sterile-filtered | |
| No HEPES, No Ribonucleosides, No Deoxyribonucleosides, No Sodium Bicarbonate | |
| Gibco™ | |
| Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture |
| Primary Rat Astrocytes | |
| Powder | |
| Standard Serum Supplementation | |
| Low Glucose, Glutamine, Phenol Red, Sodium Pyruvate | |
| cGMP-compliant under the ISO 13485 standard | |
| Room Temperature |
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.