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Gibco™ MEM, NEAA, no glutamine 
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.
Supplier: Gibco™ 10370088
Description
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This MEM is modified as follows:
With: NEAA, Phenol Red
Without: L-glutamine, HEPES
The complete formulation is available.
Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification. MEM is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle's salts.
MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Specifications
| HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts | |
| Animal Origin-free | |
| MEM (Minimum Essential Medium) | |
| Sterile-filtered | |
| Low Glucose, Phenol Red, Non Essential Amino Acids (NEAA) | |
| cGMP-compliant under the ISO 13485 standard | |
| 10 x 500 mL | |
| Storage conditions: 2°C to 8°C (protect from light) Shipping conditions: Ambient Shelf life: 24 months from date of manufacture |
| Primary Rat Astrocytes | |
| 1 X | |
| Standard Serum Supplementation | |
| Sterile-filtered | |
| No Glutamine, No HEPES, No Sodium Pyruvate | |
| Gibco™ | |
| Room Temperature |
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.