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Gibco™ Medium 199, Earle's Salts 
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells.
Supplier: Gibco™ 11150059
Description
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This Medium 199 is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Specifications
| Rat epithelial | |
| Animal Origin | |
| Liquid | |
| Sterile-filtered | |
| Low Glucose, Glutamine, Phenol Red | |
| cGMP-compliant under the ISO 13485 standard | |
| 500 mL | |
| Storage conditions: 2–8°C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
| Chick Embryo Fibroblasts, Mouse Pancreatic Epithelium, Rat Lens Tissues | |
| 1X | |
| Medium 199 | |
| Sterile-filtered | |
| No HEPES, No Sodium Pyruvate | |
| Gibco™ | |
| Room Temperature |
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.