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Gibco™ McCoy's 5A (Modified) Medium 
McCoy's 5A (modified) Medium is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues.
Supplier: Gibco™ 16600108
Description
McCoy's 5A (modified) Medium is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.
This McCoy's 5A is modified as follows:
With: High Glucose, L-glutamine, Bacto-peptone, Phenol Red
Without: Sodium Pyruvate, HEPES
The complete formulation is available.
Using McCoy's 5A (modified) Medium
Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO2 incubator. McCoy's 5A (modified) Medium requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). McCoy's 5A (modified) Medium uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Specifications
| Rat fibroblasts | |
| Animal Origin | |
| Liquid | |
| Sterile-filtered | |
| High Glucose, Glutamine, Phenol Red, Bacto-peptone | |
| cGMP-compliant under the ISO 13485 standard | |
| 10 x 500 mL |
| Biopsy Tissues | |
| 1 X | |
| McCoy's 5A Modified Medium | |
| Sterile-filtered | |
| No HEPES, No Sodium Pyruvate | |
| Gibco™ | |
| Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |